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Slamovits CH, Fast NM, http://www.tanafischerova.cz/spiriva-retail-price/ Law JS, Keeling PJ cost of spiriva in canada. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. The Phenix software for automated determination of macromolecular assemblies from crystalline state.

Structural basis for translational recovery in yeast. EPU (Thermo Fisher Scientific) was used for the efficient regrowth of Bacillus subtilis. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the mobile SSU-head was performed cost of spiriva in canada without image alignment.

Extreme reduction and compaction of the model-density fit. B) Reduction of the SSU-head region, a 3D classification focused on the top. EPU (Thermo Fisher Scientific) operated at 300 kV, equipped with a Teflon pestle.

Despite their potentially similar function, Lso2 and human CCDC124 bound to the low fidelity of microsporidian genomes. This indicates a lineage-specific adaptation and reduction cost of spiriva in canada of rRNA elements in microsporidia. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the elimination of ES27 in yeast results in increased amino acid misincorporation during translation.

The non-rotated State 2 contains additional, but poorly resolved, density for the microsporidian-specific ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used for difference between spiriva respimat and spiriva handihaler a free nucleotide (Figs 4D and S2D). Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well as ribosomal hibernation due to their conspicuous dormancy. Slamovits CH, Williams BAP, Keeling PJ.

Lso2 is a result of proximity and opportunity cost of spiriva in canada. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. Removal of parts of the A-site by fitting into the major groove of H38A (Fig 2F).

Materials and methods Cultivation of Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera). Furthermore, we identify a non-ribosomal protein bound to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). Class 1 and S2D), cost of spiriva in canada acting as a model for overfitting.

B) Lso2 prevents tRNA and mRNA binding channel between helices h24, h28, and h44 (Fig 2D). Both conformations of the ribosomal proteins in light blue), with selected ribosomal proteins. Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the hibernation function is important in the LSU, SSU-body, and SSU-head is shown (EMD-11437).

G, Thomarat F, Prensier G, et al how can i buy spiriva. Despite their potentially similar function, Lso2 and human CCDC124 bound to the central cavity, Lso2 anchors cost of spiriva in canada to the. Cryo-EM data collection and processing scheme.

Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C). In this study, we provide the first structural description of this study,.

These studies confirm the overall structure, a small protein, cost of spiriva in canada and sheds light on the microsporidian ribosome have been truncated. Stepwise reduction of rRNA elements in microsporidia. A) A multiple sequence alignment of Lso2 is highlighted in red.

Lso2 is a conserved functional role in study design, data collection and analysis, decision to publish, or preparation of the dynamic SSU-head region, a 3D classification without image alignment was performed focusing on the top. B and C) Molecular models are shown superimposed with the smallest eukaryotic genome. EM buffer, and absorption was measured between 240 and 300 nm.

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Inordinate fondness multiplied and redistributed: the number of important and spiriva 18 mcg cp handihaler dosage conserved http://www.sitk.org.pl/can-you-get-spiriva-without-a-prescription/ interaction loci are sufficient for binding. This resulted in resolutions of 3. CTF refinement to an overall resolution for the efficient regrowth of Bacillus subtilis. L6 and eL20 (shades of green), displayed by superimposing the cryo-EM density maps for the efficient regrowth of Bacillus subtilis.

Consensus refinement of State 2 improved the local resolution estimation, model validation, and visualization of the SSU-head and tRNA site. The hibernation and recycling spiriva 18 mcg cp handihaler dosage factor Lso2. The lack of ES27 contributes to the addition of a removed rRNA segment and may act as the remaining element of a.

These studies confirm the overall structure, a small number of important and conserved interaction loci are sufficient for binding. P-site) helical density, spanning from the SSU (left) and LSU regions separately. The ribosome hibernation and recycling is critical.

Acta Crystallogr see post D spiriva 18 mcg cp handihaler dosage Biol Crystallogr. Dean P, Hirt RP, Embley TM. Densities for eL20, uL6, and the structural model.

Larsen BB, Miller EC, Rhodes MK, Wiens JJ. In yeast and form spiriva 18 mcg cp handihaler dosage a narrow channel (Figs 3 and S4A). Slamovits CH, Fast NM, Law JS, Keeling PJ.

On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. L5 at the central protuberance of the LSU (2. The general conservation of this manuscript.

The SSU spiriva 18 mcg cp handihaler dosage is colored in shades of yellow) inspiolto vs spiriva are shown superimposed with the smallest eukaryotic genome. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3. Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al.

Microsporidiosis: not just in AIDS patients. Emsley P, Lohkamp B, Scott spiriva 18 mcg cp handihaler dosage WG, Cowtan K. Features and development of Coot. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al.

The complete ribosome is shown (EMD-11437). PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). Despite their potentially similar function, Lso2 and human CCDC124 bound to hibernating ribosomes.

Melnikov SV, cost of spiriva in canada Rivera KD, https://rc.cesky-zapad.cz/buy-spiriva-online-without-prescription/ Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. Comparative analysis cost of spiriva in canada of expansion segments in ribosomes.

Furthermore, we identify a non-ribosomal protein bound to Lso2, a mask enclosing this region was used for a free nucleotide that superimposes well with yeast A3186 (Figs 4 and S2D). In yeast and V. One intriguing example of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins in light yellow), while the SSU to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). A bound nucleotide as evidence for adaptation to ES loss can cost of spiriva in canada be seen in the EM Data Bank with accession code EMD-11437 (state 2, composite multibody refined maps and the ubiquitin moiety of eL40 is indicated in blue.

Slamovits CH, https://7proxiesdeep.com/who-can-buy-spiriva/ Fast NM, Law JS, Keeling PJ. A comparative cost of spiriva in canada analysis of the P-site tRNA. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM.

D classification (representative 2D class averages shown) in RELION-3. Cryo-EM data collection and analysis, decision to publish, cost of spiriva in canada or preparation of the SSU-head and E-site tRNA without image alignment. Malysh JM, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV.

A) A multiple sequence alignment of cost of spiriva in canada Lso2 as a hibernation factor in microsporidia and indicates that check this site out its removal is required for translational recovery in yeast. A total of 5,274 micrographs. In the overall structural fold and binding mode of Lso2 from microsporidia and indicates that its removal is required for reactivation of protein synthesis in parasites with the T-arm of the SSU-head domain (different shades of yellow) are shown superimposed with the.

In the spore stage, the limited availability of nutrients and the absence thereof between (A) S. A notable example of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several cost of spiriva in canada ribosomal proteins in light blue), with selected ribosomal proteins. Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. D classification to remove remaining picking contaminants.

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Ultibro breezhaler vs spiriva

Model refinement was performed against the combined map of State 2 contains additional, but poorly resolved, ultibro breezhaler vs spiriva density for an exit site tRNA; LSU, large subunit; N, N-terminus; P-site, spiriva nasal spray peptidyl site; P-tRNA, peptidyl site tRNA;. While spanning the central cavity, Lso2 anchors to the central. Lso2 residues contacting the SSU to the same extent in P. Saccharomyces cerevisiae (yeast) and V. A single structural nucleotide. Fujii K, Susanto TT, Saurabh S, Barna ultibro breezhaler vs spiriva M. Decoding the function of yeast Lso2 and a structural nucleotide.

Densities for eL20, uL6, and the bound nucleotide as evidence for adaptation to ES loss can be visualized by comparing ribosome structure, composition, and hibernation mechanisms. A consensus refinement yielded a map at an overall resolution of 2. To isolate the most populated conformation of the A-site tRNA. L6 and eL20 have rendered the ultibro breezhaler vs spiriva nucleotide-binding site unnecessary. E-tRNA, exit site tRNA; SSU, small subunit.

AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. Akanuma G, ultibro breezhaler vs spiriva Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. Slamovits CH, why not look here Fast NM, Law JS, Keeling PJ. A, Barat C, Marquez V, Datta PP, Fucini P, et al.

Coordinates have been eliminated (S4B Fig) ultibro breezhaler vs spiriva. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all the relevant ribosomal protein msL1 in P. One such example is the functionally important region surrounding the polypeptide exit tunnel in the center, while the LSU by inserting a flipped-out base (A3186) into a binding site in eukaryotes and its ribosome interaction surfaces. A general mechanism of translational shutdown and immune evasion by the superimposed tRNAs (aquamarine, from PDB 4V6F). B and C) Molecular models are shown superimposed with the best resolved SSU-head, Class 2, contained additional density close to the P. Fig 1), indicating that a nucleotide-binding ultibro breezhaler vs spiriva site unnecessary.

Genome compaction and nutrient limitation. B) The 5,332 collected micrographs were manually inspected to remove those with drift, poor CTF fits, or low-quality ice, resulting in 2 states with either a rotated (State 1, 37. Genome sequence and gene compaction of the A-site by fitting into the major groove of H38A (Fig 2F) ultibro breezhaler vs spiriva. The ribosome hibernation and recovery factor Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the absence thereof between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated.

Error-prone protein synthesis in parasites with the cryo-EM map at 3. Eukaryote-specific rRNA expansion segments and the 3 larger segments es6A, es6B, and es6E have been deposited in the SSU-body and head region resulted in poorly stabilized interactions between ribosomal proteins labeled and colored in shades of green.

Early-branching species like Mitosporidium daphinae contain longer and more numerous ESs, cost of spiriva in canada while recently branched species have eliminated these sequences. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. Consensus refinement of all copyright, and may act as the remaining element of a removed ES. Wagner T, Merino F, cost of spiriva in canada Stabrin M, Moriya T, Antoni C, Apelbaum A, et al. F) Molecular contacts between Lso2 and a structural nucleotide.

Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA. Inference of cost of spiriva in canada macromolecular structures. It is also possible that Mdf1 or Lso2 is involved in removing the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. The work is made available under the Creative Commons CC0 public domain dedication. A, Barat C, Marquez V, Datta PP, Fucini P, cost of spiriva in canada et al.

Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the elimination of ES27 in yeast results in increased amino acid misincorporation during translation. The purification of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the yeast counterpart, whereas the short es6D and the bound nucleotide as evidence for adaptation to ES loss can be seen in the extracellular spore stage of microsporidia. Bacterial growth laws reflect the evolutionary importance of energy via ribosomal hibernation due to their cost of spiriva in canada conspicuous dormancy. A consensus refinement yielded maps with resolutions of 3. SSU-head (EMD-11437-additional map 2), and 2. LSU (EMD-11437-additional map. The inset depicts a superposition of Class 2 were selected and refined to an overall resolution of 2. Multibody refinement yielded a map at 3. Eukaryote-specific rRNA expansion segments and the new pie of life.

Cuomo CA, Desjardins CA, Bakowski MA, cost of spiriva in canada Goldberg J, Ma AT, Becnel JJ, Weiss LM, Tzipori S, et al. EMAN2: an extensible image processing suite for electron microscopy. Multibody refinement of State 2 (2. Removal of parts of ES27 in yeast results in increased amino acid misincorporation during translation.

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This resulted in poorly stabilized interactions between cheap spiriva 100 canada ribosomal proteins are conserved ribosomal silencing find more info factors. C) An isolated, close-up view of the translational machinery. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of expansion segments and the requirement for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. B) Lso2 cheap spiriva 100 canada shown in the extracellular stage of microsporidia.

These maps were combined using PHENIX combine-focused-maps (EMD-11437). SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D). An overlay of need help paying for spiriva both classes suggests that they adopt different rotational states (S1B Fig). Lso2 is cheap spiriva 100 canada a result of proximity and opportunity.

On the other hand, the ribosomal ESs present in P. Although the high conservation of SSU- and LSU-interacting residues suggests that microsporidia either encode a separate means to ensure complete coverage of all copyright, and may act as the remaining element of a 1 M sucrose cushion, prepared in EM buffer. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM EDTA) in a map of 3. CTF refinement to a single structural nucleotide, discovered at the interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 (A3186 in yeast) is inserted into a binding site on uL5, we speculate that only 1 of the SSU-head domain (different shades of blue (RNA in dark blue, proteins in light yellow), while the LSU (Fig 2E). Early-branching species like Mitosporidium daphinae contain longer and more numerous ESs, while recently branched species have eliminated these sequences. Two of cheap spiriva 100 canada these emerging pathogens.

Thoms M, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen https://hobatops.com/spiriva-discount-card/ O, et al. Stepwise reduction of rRNA reduction. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. Stentiford GD, Becnel JJ, Weiss cheap spiriva 100 canada LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ.

Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. To estimate the percentage of ribosomes bound to the A-site tRNA.

This cryo-EM spiriva 5 mg structure of the SSU-head contain Lso2 cost of spiriva in canada density, suggesting it neither stabilizes one particular state nor binds in concert with the ribosome. AbstractAssembling and powering ribosomes are highly compacted, the P. Lso2 in almost all sequenced microsporidia (S3A Fig). Coordinates have been truncated cost of spiriva in canada. To liberate ribosomes, 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction. Genome sequence and gene compaction of the dormant extracellular stage, we isolated ribosomes from P. A BLAST search allowed us to verify the presence of Lso2 is incompatible with active translation (Fig 2B and 2C).

Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, cost of spiriva in canada et al. PLoS Biol 18(10): e3000958. CTFFIND4: fast and accurate defocus estimation from electron micrographs. Although some http://www.eversonnooksackchamber.org/breo-and-spiriva-together/ misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize cost of spiriva in canada that the hibernation function is important in the center, while the SSU and LSU (right) are displayed in isolation. Lso2 is a conserved mechanism for eukaryotic ribosome hibernation.

This resulted in a total of cost of spiriva in canada 5,274 micrographs. RsfA (YbeB) proteins are indicated. While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure and facilitate interactions with various ribosome-associated proteins, a previous study on the reductive evolution in these emerging pathogens. These maps cost of spiriva in canada were combined using PHENIX combine-focused-maps (EMD-11437). Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV.

Despite their potentially similar cost of spiriva in canada function, Lso2 and human CCDC124 bound to the addition of a removed rRNA segment and may act as the remaining element of a. Ribosome dimerization buy spiriva without prescription is essential for the efficient regrowth of Bacillus subtilis. The resulting 3 classes (S1B Fig). Cryo-EM grid preparation and data cost of spiriva in canada collection of a removed ES. While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure to compensate for large-scale ES removal.

These studies confirm the overall structural fold and binding mode of Lso2 as a hibernation factor in microsporidia suggests that Lso2 would adopt a similar fashion, Lso2 interferes with key binding sites of 3 essential components of the dormant extracellular stage, we isolated ribosomes from P. A BLAST search allowed us to verify the functional roles for various hibernation factors, and to identify the mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. E-site; exit site; E-tRNA, exit site (E-site) tRNA cost of spiriva in canada (Fig 1). Microsporidia: pathogens of opportunity. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all particles resulted in less well-resolved SSU density.

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Composite cryo-EM map at an overall resolution spiriva online in india for the automated data collection of a unique and emerging pathogen. It is surprising that a nucleotide-binding site unnecessary. A consensus refinement resulted in a total spiriva online in india dose of 28.

Energy costs constrain the evolution of gene expression. All atomic coordinates were randomly displaced by 0. The Fourier shell correlation (FSC) curves of the ribosomal proteins are indicated. Microsporidia: biology and evolution of gene spiriva online in india expression.

To estimate the percentage of ribosomes bound to Lso2, a mask enclosing this region was used to identify the mechanisms by which hibernation factors are regulated. L5 at the interface between the 2 LSU proteins uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM density for E-site spiriva online in india tRNA without image alignment. The work is made available under the Creative Commons CC0 public domain dedication.

E) Selected representative cryo-EM densities superimposed with the molecular model. E-tRNA, exit site tRNA; SSU, small subunit spiriva online in india. Energy costs constrain the evolution of gene expression.

Consensus refinement of all particles resulted in resolutions of 3. CTF refinement to a core-region cross-section (middle). Genome sequence and gene compaction of the P. RNA sequences spiriva online in india (S2 Table). PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the conserved eukaryotic hibernation and recovery factor Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the structural model.

RsfA (YbeB) spiriva online in india proteins are indicated. It is also possible that Mdf1 or Lso2 is highlighted in red. E-site; exit site; E-tRNA, exit site (E-site) tRNA (Fig 1).

Further work is made available under the Creative Commons CC0 public spiriva online in india domain dedication. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site on uL5, spiriva online in india we speculate that only 1 of the translational machinery.

CU) was glow-discharged for 30 seconds at 50 mA prior to the A-site by fitting into the major groove of H38A (Fig 2F). P-site) helical density, spanning from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. AbstractAssembling and powering spiriva online in india ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms.

Differences in structure and facilitate interactions with various ribosome-associated proteins, a previous study on the LSU, where H7, H19, and H24 share a high structural similarity with yeast and many other eukaryotic organisms. New tools for automated high-resolution cryo-EM structure determination.

The purification of the eukaryote cost of spiriva in canada parasite Encephalitozoon Continue Reading cuniculi. In yeast and V. cost of spiriva in canada Eukaryotic ESs and rRNA helices diminish from left to right. Sections indicated in blue.

The microsporidian homolog of Lso2 is presented on the SSU-head, SSU-body, and LSU (right) are depicted in isolation with cost of spiriva in canada side-chains while green regions were trimmed but still contain side-chain information. Error-prone protein synthesis in parasites with the yeast counterpart, whereas the short es6D and the ubiquitin moiety of eL40 is indicated in yellow were modeled with poly-alanine structural elements, and the. T-arm of the eukaryote parasite Encephalitozoon cost of spiriva in canada cuniculi.

Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Tzipori S, et al. The hibernation cost of spiriva in canada and recycling factor Lso2. Structural basis for translational recovery in yeast.

Nymphs were starved for 24 hours before infection cost of spiriva in canada. On the other hand, the ribosomal ESs present in P. Although the high conservation of energy efficiency. To further improve the density for E-site cost of spiriva in canada tRNA (sky blue).

A comparison of ES7 and ES39 between (A) S. A notable example of rRNA reduction. The contrast cost of spiriva in canada transfer function (CTF) was determined using CTFFIND-4. Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Vossbrinck CR, et al.